SP4 is a subproject within a DFG-funded research initiative focused on examining the interactions between pollutants, antibiotic resistance, and pathogens in an evolving wastewater system. The research specifically investigates how irrigation water quality impacts the soil and plant-associated microbiome, along with the abundance, diversity, and transferability of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) in Gram-positive bacteria.